Monocyte Chemotactic and Activating Factor/Monocyte Chemoattractant Protein 1 (MCAF/MCP1) in bronchoalveolar lavage fluid (BAL-f) in patients with asthma and chronic bronchitis

Karina Jahnz-Rozyk1, Piotr Kuna2, Tadeusz Plusa1, Ewa Pirozynska1

  1. II Department of Internal Medicine of Military Academy in Warsaw, Poland
  2. Clinical Department of Pneumonology and Allergy of Medical Academy in Lodz, Poland

Published in: R. Spiewak (Editor): "Pollens and Pollinosis: Current Problems". Institute of Agricultural Medicine, Lublin (Poland) 1995, page 92.

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Polski Po Polsku

MCAF/MCP1 belongs to beta-subgroup of chemokines. Chemokines are cytokine-like molecules that function as chemotactic factors. They have a role in the recruitment and degranulation of basophils, but also in activation of eosinophils, monocytes and lymphocytes. That means they may be very important factors in evoking of inflammatory changes in human airways.

Objective

Evaluation of MCAF/MCP1 concentration in BAL-f from patients with bronchial asthma and chronic bronchitis.

Material

16 patients (7 women and 9 men) aged 22 +/- 4 with atopic bronchial asthma (pollinosis), 10 patients (3 women and 7 men) aged 40 +/- 3 with chronic bronchitis and 4 healthy people (2 women and 2 men) aged 35 +/- 3 of control group took part in our study.

Methods

BAL was done in routine way and then BAL-f cytology including viability and amount of cells was evaluated in every patient. ELISA test using mono- and polyclonal antibodies was used to determine the concentration of MCAF/MCP1.

Results

MCAF/MCP1 concentration (pg/ml) was: 156.4 +/- 92 in patients with atopic asthma, 335 +/- 137 in patients with chronic bronchitis and 82.2 +/- 11.6 in control group (p<0.05).

Conclusion

  1. The concentration of MCAF/MCP is increased in patients with asthma and chronic bronchitis compared to control group.
  2. The highest concentration of MCAF/MCP1 was found in patients with chronic bronchitis.
  3. Monitoring of MCAF/MCP1 activity in BAL-f can be useful in assessment of inflammatory changes in human airways.

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